Morales Ovalle, Marco A. (2021) Optimización del tamaño de nanopartículas magnéticas de MnFe2O4 para aplicaciones conjuntas de hipertermia y producción de radicales libres en terapias oncológicas / Size optimazation of MnFe204 magnetic nanoparticles for joint applications of hyperthermia and free radicals production in cancer therapies. Maestría en Física Médica, Universidad Nacional de Cuyo, Instituto Balseiro.
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Resumen en español
Según la Organización Mundial de la Salud el cáncer es una de las principales causas de morbilidad y mortalidad en el mundo. Diferentes tratamientos han sido utilizados para su control, pero todos acarrean cierto nivel de riesgo o efectividad. La hipertermia entre ellos es un tratamiento basado en el incremento de temperatura a niveles críticos para la viabilidad celular. Se ha observado que las nanopartículas magnéticas bajo la in fluencia de un campo magnético alterno pueden generar calor, incrementando la temperatura a escala macroscópica, por lo que se ha estudiado su efectividad para tratamientos de hipertermia. A su vez, se ha reportado muerte celular significativa a pesar de la ausencia de un incremento de la temperatura en presencia de nanopartículas magnéticas de óxido de hierro. Se ha propuesto que el efecto es debido al incremento de temperatura a escala nanométrica, o bien, debido al efecto de las nanopartículas magnéticas como catalizadores en la producción de radicales libres. Para este trabajo se sintetizaron y caracterizaron nanopartículas magnéticas de ferrita de manganeso monodispersas con diámetros de 12 y 28 nm. Se evaluó su eficiencia como agentes de calentamiento y su capacidad para catalizar la producción de radicales libres, obteniendo valores de absorción de potencia entre 13-460 W/g y concentraciones de .OH de hasta 2.3 µM. Por lo tanto, decidimos estudiar el comportamiento de las nanopartículas en la línea de células hepáticas HepG2. Se determinó una disminución de la viabilidad celular respecto del control, previo a la aplicación del tratamiento con un campo magnético. A pesar de no alcanzar un incremento perceptible de la temperatura, se avaluó el efecto del campo debido a las nanopartículas, sin embargo, no se encontró una diferencia significativa entre la viabilidad observada antes y después de la exposición de las celulas al campo. A su vez se analizó el grado de peroxidación lipídica como consecuencia de estrés oxidativo a través del ensayo de las sustancias reactivas del ácido tiobarbitúrico (TBARS) y se encontró un aumento significativo respecto del control. Esto parece indicar que el detrimento celular pudo tener lugar por estrés oxidativo. Por otro lado, la aplicación de un campo magnético alterno tampoco modificó el contenido de TBARS.
Resumen en inglés
According to the World Health Organization, cancer is one of the main causes of morbidity and mortality in the world. Different treatments have been used to control it, but all carry a certain level of risk or effectiveness. Hyperthermia among them is a treatment based on increasing the temperature up to critical levels for cell viability. It has been observed that magnetic nanoparticles under the in uence of an alternating magnetic eld can generate heat, increasing the temperature on a macroscopic scale, for which their effectiveness for hyperthermia treatments has been studied. In turn, signicant cell death has been reported despite the absence of a measurable temperature change in the presence of magnetic iron oxide nanoparticles. It has been proposed that this effect is due to the increase in temperature on a nanometric scale, or else due to the effect of magnetic nanoparticles as catalysts in the free radicals production. For this work, monodisperse manganese ferrite magnetic nanoparticles with diameters of 12 and 28 nm were synthesized and characterized. Their effciency as heating agents and their ability to catalyze the free radicals production were evaluated, obtaining power absorption values between 13-460 W/g and concentrations of .OH up to 2.3 µM. Therefore, we decided to study nanoparticle behavior on HepG2 liver cell line. A decrease in cell viability was determined with respect to the control, prior to the application of the magnetic eld treatment. Despite not achieving a perceptible increase in temperature, the effect of the eld due to the nanoparticles was evaluated; however, no signicant difference was found between the viability observed before and after the exposure of the cells to the eld. In turn, the degree of lipid peroxidation was analyzed as a consequence of oxidative stress through the test of reactive substances of thiobarbituric acid (TBARS) and a signicant increase was found compared to the control. This seems to indicate that the cellular detriment could have been due to oxidative stress. On the other hand, the application of an alternating magnetic eld did not modify the TBARS content either.
| Tipo de objeto: | Tesis (Maestría en Física Médica) |
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| Palabras Clave: | Hyperthermia; Hipertermia; Free radicals; Radicales libres; Peroxidases; Peroxidasas; [Magnetic nanoparticles; Nanopartículas magnéticas; Specific potencial absorption; Absorción específica de potencia] |
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| Materias: | Medicina > Física médica |
| Divisiones: | Gcia. de área de Investigación y aplicaciones no nucleares > Gcia. de Física > Ciencias de materiales > Resonancias magnéticas |
| Código ID: | 955 |
| Depositado Por: | Marisa G. Velazco Aldao |
| Depositado En: | 30 Jul 2021 09:42 |
| Última Modificación: | 30 Jul 2021 09:42 |
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